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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is using Sanger sequencing to identify mutations associated with antibiotic resistance in Mycobacterium tuberculosis. This method directly determines the:

A) Nucleotide sequence of the target gene.
B) Relative abundance of different M. tuberculosis strains in a mixed infection.
C) Presence or absence of specific resistance genes.
D) Minimum inhibitory concentration (MIC) of the antibiotic.

2. A clinical microbiology laboratory is using a nucleic acid amplification test (NAAT) to detect Mycobacterium tuberculosis directly from sputum. A positive result indicates:

A) Active tuberculosis disease requiring immediate treatment.
B) The presence of M. tuberculosis DNA, which could be from viable or non-viable organisms.
C) Drug susceptibility information for the M. tuberculosis strain.
D) The presence of viable M. tuberculosis organisms.

3. A clinical microbiology laboratory encounters a Gram-negative bacillus isolated from a bloodstream infection. The isolate exhibits resistance to multiple antibiotics, including carbapenems. Further phenotypic testing reveals the production of a metallo-beta-lactamase (MBL). Which of the following antibiotic combinations might still retain activity against this isolate?

A) Ceftazidime-avibactam
B) Piperacillin-tazobactam
C) Aztreonam
D) Aztreonam-avibactam

4. A microbiology laboratory receives a corneal scraping from a patient with keratitis. Microscopic examination with calcofluor white stain reveals the presence of septate hyphae and conidiophores with a foot cell and phialides. The MOST likely genus is:

A) Mucor
B) Aspergillus
C) Rhizopus
D) Candida

5. A clinical microbiology laboratory is validating a new multiplex PCR assay for the simultaneous detection of several gastrointestinal pathogens, including Salmonella, Shigella, and Campylobacter. To ensure the accuracy and reliability of the assay, it is crucial to include appropriate controls. Which of the following sets of controls would be MOST comprehensive?

A) Positive controls for each target organism, a negative control without template DNA, and an internal amplification control.
B) Positive controls for each target organism, a negative control without template DNA, an internal amplification control, and a process control to monitor nucleic acid extraction.
C) Positive controls for each target organism and a negative control without any template DNA.
D) Positive controls for each target organism, a negative control containing non-target DNA, and a process control to monitor nucleic acid extraction.

Solutions: